Welcome to the BBSRC Single Cell Sequencing Platform

Contact us at: [email protected]

 

The role of this platform is to provide state of art technologies to aid academic and industrial partners in a wide range of sequencing applications.

Housed in the Biomolecular Sciences building at North Haugh, this platform consists of:

  • Agilent 4200 TapeStation System – for quality control of sequencing libraries and samples
  • 10X Genomics Chromium X Controller – for single cell sorting and barcoding
  • Illumina NextSeq1000 – for generation of DNA and RNA sequencing data

 

The platform is opened for both internal and external studies. We are here to assist and truly accelerate your research.

 

For all enquiries, including quotes and help with grant costing, please contact:
[email protected]

 

Image of the NextSeq 1000 sequencer. This sequencer is a large silver cube with a wide strip of LED lighting around the centre of the instrument, currently display white and purple colouring. The instrument has a tablet on the front of it, displaying a white screen and the word 'start'.
NextSeq 1000
Image of the Chromium X fluidics device used for single cell sorting. This device is a cuboid that is black on the top and white on the bottom. A tray is ejected from the machine. A display screen of the front of the device shows an eject button.
Chromium X
Image of the 4200 TapeStation used for quality control of samples and sequencing libraries. This machine is a large white cube with a lid that opens upwards. The lid is open in this image showing the inside of the machine.
4200 TapeStation

 

What is Next Generation Sequencing?

Technology that elevated and accelerated genetic research since Sanger Sequencing method was developed.

Novel fluorescent-based sequencing method.

First Next Gene Sequencers were commercially available in early 2000s and revolutionized biomedical research by allowing ’’massive parallel sequencing’’ of any purified and prepared genetic material giving wide range of applications.

Nowadays, NGS allows cost effective, high speed and automated nucleotide sequence determination with just a small sample .

What is Single Cell Sequencing?

Single-cell sequencing is an elevation to NGS where single cell assay techniques are combined with fluorescence based sequencing for insight into differences within typically genetically undistinguishable cellular populations. Here, genetic material from cells can be sequenced to allow genomic, transcriptomic or multi-omic profiling at single cell level.

 
 
BBSRC Single Cell Sequencing Platform
Biomolecular Sciences Building, North Haugh
St Andrews, Fife, KY16 9ST
 
 

Get in touch with our team via email or join our sequencing chatroom in person to find out more!